Role of calcineurin in airway remodeling in guinea pig model of asthma

AIM:To investigate the role of calcineurin (CaN) in airway remodeling in guinea pig model of asthma.METHODS:Male guinea pigs were randomly divided into three groups: control, asthma group and CsA group. The following parameters were measured: 1. The protein content, cell count and differential count of BALF; 2. The amount of [³H]-TdR incorporation into central airway smooth muscle; 3. The mean thickness of airway wall and airway smooth muscle of small airwaysl; 4.CaN activity of trachea and lung tissue.RESULTS:1. The protein content, cell count and eosinophil of BALF in CsA group were 46%, 51% and 60% lower than those in asthma group, respectively (P＜0.01); 2. [³H]-TdR incorporation in CsA group was 22% lower than that in asthma group (P＜0.05);3. The mean thickness of airway wall and airway smooth muscle were 34% and 37% less in CsA group than those in asthma group, respectively (P＜0.01); 4. CaN activity of lung tissue and trachea were 52% and 44% lower in CsA group than those in asthma group, respectively (P＜0.01).CONCLUSION:CsA reduced airway remodeling in guinea pig model of asthma, indicating the role of CaN in the airway remodeling.     

Expression of alpha-smooth muscle actin in scar tissue and its relationship with apoptosis

AIM: To examine the expression of alpha-smooth muscle actin in scar tissue, and observe the phenomenon of apoptosis and its involvement in the process of pathological scarring and the presence of myofibroblasts or absence of cell in the dermis. To investigate the potential role of reparative cell apoptosis in hyperplastic scar formation. METHODS: The samples of scar were obtained from post-burn patients undergoing plastic operation in our burn unit recently, and the samples of control came from skin donor site of the same patient correspondingly. TUNEL assays were performed to evaluate the number of apoptotic cells in scar versus normal skin. In situ hybridization and immunohistochemistry staining technique were employed to determine the expression of different dermis cells markers in scar tissue and normal skin. RESULTS: There existed evident difference in apoptotic cells in the dermis between scars tissue and normal human skin. The expression positive cells were much more in hyperplastic scars than that in normal human skin; the apoptotic cells of proliferative stage were slight more than that of mature stage. However, in proliferative stage, the number of apoptotic cells was higher for the combination of hyperplastic scar than normally healed flat scars. But in mature stage, no obviously difference was detected between hyperplastic scar and normally healed flat scar. The monoclonal anti-α smooth muscle actin (ASMA) expression was significantly stronger in proliferative stage than that of mature stage. CONCLUSIONS: With reconstitution of dermal tissue, myofibroblasts containing alpha-SM actin disappear under normal wound healing, probably as a result of apoptosis. The myofibroblast play a critical role in wound closure and in the pathologic sequelae of healing.     

兽用抗寄生虫药物新剂型及其新技术的研究进展

兽用抗寄生虫药物由于使用频繁,其给药技术研究一直备受重视.目前,兽用抗寄生虫药物新剂型和新技术的研究主要集中在缓释控释制剂和脂质体制剂,其次是透皮给药系统、微囊、微球、环糊精包合物、固体分散体等.但这些新剂型和新技术大多还处于研究阶段,在处方设计或制剂工艺方面都还存在一些问题,今后应加强这些新剂型的工艺和技术研究,以真正发挥这些新剂型的优点.     

离子对高效液相色谱法测定复方甲硝唑片中甲硝唑和维生素B6含量

建立了同时测定复方甲硝唑片中甲硝唑和维生素B6含量的离子对HPLC方法.采用ODS色谱柱,流动相为磷酸二氢钾和庚烷磺酸钠水溶液(每1L中含磷酸二氢钾13.6 g与庚烷磺酸钠1.0 g)-甲醇-三乙胺(85∶15∶0.4),用磷酸调pH至2.8;检测波长290 nm,以峰面积计算,外标法定量.甲硝唑和维生素B6的线性范围分别为100～300 μg/mL(r=0.999 9)和5～50 μg/mL(r=0.999 9);平均回收率分别为100.2%(RSD=1.0%,n=6),99.8%(RSD=1.2%,n=6).该方法测定结果准确,操作简便,回收率高.     

外来入侵种的危害与防治对策

外来入侵种对当地的自然生态系统结构和功能以及生物多样性都有着深远的影响,而且可能对地方的生态环境和经济造成严重危害。四川已经成为遭受外来入侵种威胁的省份之一,全省现有20多个外来入侵种。为了防止外来入侵种的泛滥,应当建立外来物种的引入评价机制,慎重进行有意引种;加强口岸管理,防止外来入侵种通过贸易途径无意引入,同时采取措施控制已经产生危害的外来入侵种。     

Nitric oxide-mediated the cardioprotection of tumor necrosis factor-alpha on cultured neonatal rat cardiomyocytes during hypoxia/reoxygenation

AIM: To investigate the role of nitric oxide synthase (NOS), soluble guanylyl cyclase (sGC) and protein kinase C (PKC) signaling in tumor necrosis factor-α (TNF-α)-induced cardioprotection against hypoxia/reoxygenation (H/R) injury. METHODS: Neonatal rat ventricular myocytes were pretreated with TNF-α or sodium nitroprusside (SNP) or L-arginine (L-Arg), respectively, for 12 h and then subjected to continuous hypoxia for 12 h, followed by reoxygenation for 6 h. The manganese superoxide dismutase (Mn-SOD) activity of the cells was measured after H/R. Myocyte injury was determined by the release of lactic dehydrogenase (LDH). RESULTS: TNF-α (10⁵ U/L) significantly increased the Mn-SOD activity and decreased release of LDH from ventricular myocytes. The cardioprotection against H/R injury was induced by the pretreatment with SNP (5 μmol/L) or L-Arg (5 mmol/L), which was blocked by ODQ (10 μmol/L), the specific sGC inhibitor, and Chel (5 μmol/L), the specific PKC inhibitor. Pretreatment with L-NAME (100 μmol/L), ODQ, Chel, antoxidant 2-MPG (400 μmol/L) or tyrosine kinase inhibitor genistein (50 μmol/L) attenuated the increased Mn-SOD activity and reduced LDH level induced by TNF-α. CONCLUSION: The results suggest that NO may play a role in TNF-α-induced cardioprotection, which is mediated by sGC and PKC.     

蜱传性疾病--莱姆病螺旋体研究进展

莱姆病是一种蜱传性人兽共患病,在我国北方各省广泛流行,其病原体为伯氏疏螺旋体。莱姆病可使皮肤、心脏、关节和神经系统等多种组织器官受损。本文综述了莱姆病螺旋体的形态、种下分类、生化特征、传播媒介和贮存宿主等方面的研究进展"     

犬传染性喉气管炎病毒的分离及鉴定

用犬肾细胞 (MDCK)从疑似犬传染性喉气管炎病毒感染犬的急性期血清中分离到 1株病毒 ,经血凝试验、免疫电镜观察、中和试验、免疫荧光抗体试验、细胞培养特征观察和回归动物试验等鉴定 ,证明所分离的病毒为犬传染性喉气管炎病毒 ,命名为 BJ- JB- 3。     

2个旋毛虫新生幼虫期特异性相似cDNA的克隆及序列分析

利用旋毛虫新生幼虫期特异性 c DNA探针 ,从新生幼虫 c DNA文库中筛选出 2个相似的旋毛虫新生幼虫期特异性 c DNA克隆 ,分别命名为 N5和 N10 ,N5长度为 12 5 0 bp,N10长度为 12 33bp。 NCBI Blast检索表明 ,2个c DNA全长序列均为旋毛虫新基因序列。DNASIS分析表明 ,N5与 N10的开放阅读框架分别为 10 14、10 17bp,编码338、339个氨基酸 ,推导的成熟蛋白氨基酸序列均为 32 1个氨基酸残基 ,相对分子质量推导值分别为 35 30 0和 354 0 0。 2个氨基酸序列中 N端均包含有一信号肽序列 ,可能为分泌性蛋白。NCBI Blast及 Inter Proscan检索表明 ,以上2个氨基酸序列均含有 型核酸酶的功能结构域 ,均编码 型核酸酶 (DNase ) ,且与已报道的旋毛虫包囊形成相关蛋白 P4 3(经鉴定也为 DNase )同源性最高。目前已有的试验结果证实 ,P4 3并未直接参与包囊的形成 ,而是一种与P4 3蛋白同源性非常高的蛋白参与了旋毛虫包囊的形成。由于 N5与 N10为旋毛虫新生幼虫期特异性表达基因 ,也就是在旋毛虫包囊形成的时期表达 ,而且与 P4 3具有较高的同源性 ,并均编码 DNase 蛋白 ,因此 N5、N10具有参与旋毛虫包囊形成的潜在可能     

夏季狗牙根草坪不同建植方法及秋季交播黑麦草对成坪效果的影响

以狗牙根Cynodon dactylon种子和营养体为材料，在武汉地区，对5种不同建植方法建成的草坪从盖度变化、成坪时间、均一性、建植成本及秋季交播多年生黑麦草Lolium perenne后翌年狗牙根的草坪质量等方面进行综合比较。结果表明：采用夏季建植狗牙根草坪并于秋季交播多年生黑麦草的方法来建植四季常绿草坪，狗牙根采用撒茎覆沙法效果最佳，其他方法依次为开沟埋茎法、闻铺草皮法、穴植草茎法；种子直播法在翌年需重新建坪。